Identification of differentially expressed proteins in poplar leaves induced by Marssonina brunnea f. sp. Multigermtubi.
Identifieur interne : 003895 ( Main/Exploration ); précédent : 003894; suivant : 003896Identification of differentially expressed proteins in poplar leaves induced by Marssonina brunnea f. sp. Multigermtubi.
Auteurs : Kun Yuan [République populaire de Chine] ; Bo Zhang ; Yanmei Zhang ; Qiang Cheng ; Mingxiu Wang ; Minren HuangSource :
- Journal of genetics and genomics = Yi chuan xue bao [ 1673-8527 ] ; 2008.
Descripteurs français
- KwdFr :
- Feuilles de plante (microbiologie), Feuilles de plante (métabolisme), Immunité innée (MeSH), Maladies des plantes (microbiologie), Populus (microbiologie), Populus (métabolisme), Protéines végétales (analyse), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Spectrométrie de masse MALDI (MeSH), Électrophorèse bidimensionnelle sur gel (MeSH).
- MESH :
- analyse : Protéines végétales.
- microbiologie : Feuilles de plante, Maladies des plantes, Populus.
- métabolisme : Feuilles de plante, Populus, Protéines végétales.
- Immunité innée, Régulation de l'expression des gènes végétaux, Spectrométrie de masse MALDI, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- Electrophoresis, Gel, Two-Dimensional (MeSH), Gene Expression Regulation, Plant (MeSH), Immunity, Innate (MeSH), Plant Diseases (microbiology), Plant Leaves (metabolism), Plant Leaves (microbiology), Plant Proteins (analysis), Plant Proteins (metabolism), Populus (metabolism), Populus (microbiology), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH).
- MESH :
- chemical , analysis : Plant Proteins.
- metabolism : Plant Leaves, Plant Proteins, Populus.
- microbiology : Plant Diseases, Plant Leaves, Populus.
- Electrophoresis, Gel, Two-Dimensional, Gene Expression Regulation, Plant, Immunity, Innate, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
Black spot disease in poplar is a disease of the leaf caused by fungus. The major pathogen is Marssonina brunnea f. sp. multigermtubi. To date, little is known about the molecular mechanism of poplar (M. brunnea) interaction. In order to identify the proteins related to disease resistance and understand its molecular basis, the clone "NL895" (P. euramericana CL"NL895"), which is highly resistant to M. brunnea f. sp. multigermtubi, was used in this study. We used two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to identify the proteins in poplar leaves that were differentially expressed in response to black spot disease pathogen, M. brunnea f. sp. multigermtubi. Proteins extracted from poplar leaves at 0, 12, 24, 48, and 72 h after pathogen-inoculation were separated by 2-DE. About 500 reproducible protein spots were detected, of which 40 protein spots displayed differential expression in levels and were subjected to Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) followed by database searching. According to the function, the identified proteins were sorted into five categories, that is, protein synthesis, metabolism, defense response and unclassified proteins.
DOI: 10.1016/S1673-8527(08)60007-7
PubMed: 18222409
Affiliations:
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The major pathogen is Marssonina brunnea f. sp. multigermtubi. To date, little is known about the molecular mechanism of poplar (M. brunnea) interaction. In order to identify the proteins related to disease resistance and understand its molecular basis, the clone "NL895" (P. euramericana CL"NL895"), which is highly resistant to M. brunnea f. sp. multigermtubi, was used in this study. We used two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to identify the proteins in poplar leaves that were differentially expressed in response to black spot disease pathogen, M. brunnea f. sp. multigermtubi. Proteins extracted from poplar leaves at 0, 12, 24, 48, and 72 h after pathogen-inoculation were separated by 2-DE. About 500 reproducible protein spots were detected, of which 40 protein spots displayed differential expression in levels and were subjected to Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) followed by database searching. According to the function, the identified proteins were sorted into five categories, that is, protein synthesis, metabolism, defense response and unclassified proteins.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18222409</PMID><DateCompleted><Year>2009</Year><Month>04</Month><Day>24</Day></DateCompleted><DateRevised><Year>2008</Year><Month>01</Month><Day>28</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1673-8527</ISSN><JournalIssue CitedMedium="Print"><Volume>35</Volume><Issue>1</Issue><PubDate><Year>2008</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of genetics and genomics = Yi chuan xue bao</Title><ISOAbbreviation>J Genet Genomics</ISOAbbreviation></Journal><ArticleTitle>Identification of differentially expressed proteins in poplar leaves induced by Marssonina brunnea f. sp. 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About 500 reproducible protein spots were detected, of which 40 protein spots displayed differential expression in levels and were subjected to Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) followed by database searching. 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